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neurokit2

🎯Skill

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What it does

Processes and analyzes diverse physiological signals like ECG, EEG, EDA, and EMG to extract complex cardiovascular, neurological, and autonomic nervous system metrics for research and clinical appl...

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neurokit2

Installation

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AddedFeb 4, 2026

Skill Details

SKILL.md

Comprehensive biosignal processing toolkit for analyzing physiological data including ECG, EEG, EDA, RSP, PPG, EMG, and EOG signals. Use this skill when processing cardiovascular signals, brain activity, electrodermal responses, respiratory patterns, muscle activity, or eye movements. Applicable for heart rate variability analysis, event-related potentials, complexity measures, autonomic nervous system assessment, psychophysiology research, and multi-modal physiological signal integration.

Overview

# NeuroKit2

Overview

NeuroKit2 is a comprehensive Python toolkit for processing and analyzing physiological signals (biosignals). Use this skill to process cardiovascular, neural, autonomic, respiratory, and muscular signals for psychophysiology research, clinical applications, and human-computer interaction studies.

When to Use This Skill

Apply this skill when working with:

  • Cardiac signals: ECG, PPG, heart rate variability (HRV), pulse analysis
  • Brain signals: EEG frequency bands, microstates, complexity, source localization
  • Autonomic signals: Electrodermal activity (EDA/GSR), skin conductance responses (SCR)
  • Respiratory signals: Breathing rate, respiratory variability (RRV), volume per time
  • Muscular signals: EMG amplitude, muscle activation detection
  • Eye tracking: EOG, blink detection and analysis
  • Multi-modal integration: Processing multiple physiological signals simultaneously
  • Complexity analysis: Entropy measures, fractal dimensions, nonlinear dynamics

Core Capabilities

1. Cardiac Signal Processing (ECG/PPG)

Process electrocardiogram and photoplethysmography signals for cardiovascular analysis. See references/ecg_cardiac.md for detailed workflows.

Primary workflows:

  • ECG processing pipeline: cleaning β†’ R-peak detection β†’ delineation β†’ quality assessment
  • HRV analysis across time, frequency, and nonlinear domains
  • PPG pulse analysis and quality assessment
  • ECG-derived respiration extraction

Key functions:

```python

import neurokit2 as nk

# Complete ECG processing pipeline

signals, info = nk.ecg_process(ecg_signal, sampling_rate=1000)

# Analyze ECG data (event-related or interval-related)

analysis = nk.ecg_analyze(signals, sampling_rate=1000)

# Comprehensive HRV analysis

hrv = nk.hrv(peaks, sampling_rate=1000) # Time, frequency, nonlinear domains

```

2. Heart Rate Variability Analysis

Compute comprehensive HRV metrics from cardiac signals. See references/hrv.md for all indices and domain-specific analysis.

Supported domains:

  • Time domain: SDNN, RMSSD, pNN50, SDSD, and derived metrics
  • Frequency domain: ULF, VLF, LF, HF, VHF power and ratios
  • Nonlinear domain: PoincarΓ© plot (SD1/SD2), entropy measures, fractal dimensions
  • Specialized: Respiratory sinus arrhythmia (RSA), recurrence quantification analysis (RQA)

Key functions:

```python

# All HRV indices at once

hrv_indices = nk.hrv(peaks, sampling_rate=1000)

# Domain-specific analysis

hrv_time = nk.hrv_time(peaks)

hrv_freq = nk.hrv_frequency(peaks, sampling_rate=1000)

hrv_nonlinear = nk.hrv_nonlinear(peaks, sampling_rate=1000)

hrv_rsa = nk.hrv_rsa(peaks, rsp_signal, sampling_rate=1000)

```

3. Brain Signal Analysis (EEG)

Analyze electroencephalography signals for frequency power, complexity, and microstate patterns. See references/eeg.md for detailed workflows and MNE integration.

Primary capabilities:

  • Frequency band power analysis (Delta, Theta, Alpha, Beta, Gamma)
  • Channel quality assessment and re-referencing
  • Source localization (sLORETA, MNE)
  • Microstate segmentation and transition dynamics
  • Global field power and dissimilarity measures

Key functions:

```python

# Power analysis across frequency bands

power = nk.eeg_power(eeg_data, sampling_rate=250, channels=['Fz', 'Cz', 'Pz'])

# Microstate analysis

microstates = nk.microstates_segment(eeg_data, n_microstates=4, method='kmod')

static = nk.microstates_static(microstates)

dynamic = nk.microstates_dynamic(microstates)

```

4. Electrodermal Activity (EDA)

Process skin conductance signals for autonomic nervous system assessment. See references/eda.md for detailed workflows.

Primary workflows:

  • Signal decomposition into tonic and phasic components
  • Skin conductance response (SCR) detection and analysis
  • Sympathetic nervous system index calculation
  • Autocorrelation and changepoint detection

Key functions:

```python

# Complete EDA processing

signals, info = nk.eda_process(eda_signal, sampling_rate=100)

# Analyze EDA data

analysis = nk.eda_analyze(signals, sampling_rate=100)

# Sympathetic nervous system activity

sympathetic = nk.eda_sympathetic(signals, sampling_rate=100)

```

5. Respiratory Signal Processing (RSP)

Analyze breathing patterns and respiratory variability. See references/rsp.md for detailed workflows.

Primary capabilities:

  • Respiratory rate calculation and variability analysis
  • Breathing amplitude and symmetry assessment
  • Respiratory volume per time (fMRI applications)
  • Respiratory amplitude variability (RAV)

Key functions:

```python

# Complete RSP processing

signals, info = nk.rsp_process(rsp_signal, sampling_rate=100)

# Respiratory rate variability

rrv = nk.rsp_rrv(signals, sampling_rate=100)

# Respiratory volume per time

rvt = nk.rsp_rvt(signals, sampling_rate=100)

```

6. Electromyography (EMG)

Process muscle activity signals for activation detection and amplitude analysis. See references/emg.md for workflows.

Key functions:

```python

# Complete EMG processing

signals, info = nk.emg_process(emg_signal, sampling_rate=1000)

# Muscle activation detection

activation = nk.emg_activation(signals, sampling_rate=1000, method='threshold')

```

7. Electrooculography (EOG)

Analyze eye movement and blink patterns. See references/eog.md for workflows.

Key functions:

```python

# Complete EOG processing

signals, info = nk.eog_process(eog_signal, sampling_rate=500)

# Extract blink features

features = nk.eog_features(signals, sampling_rate=500)

```

8. General Signal Processing

Apply filtering, decomposition, and transformation operations to any signal. See references/signal_processing.md for comprehensive utilities.

Key operations:

  • Filtering (lowpass, highpass, bandpass, bandstop)
  • Decomposition (EMD, SSA, wavelet)
  • Peak detection and correction
  • Power spectral density estimation
  • Signal interpolation and resampling
  • Autocorrelation and synchrony analysis

Key functions:

```python

# Filtering

filtered = nk.signal_filter(signal, sampling_rate=1000, lowcut=0.5, highcut=40)

# Peak detection

peaks = nk.signal_findpeaks(signal)

# Power spectral density

psd = nk.signal_psd(signal, sampling_rate=1000)

```

9. Complexity and Entropy Analysis

Compute nonlinear dynamics, fractal dimensions, and information-theoretic measures. See references/complexity.md for all available metrics.

Available measures:

  • Entropy: Shannon, approximate, sample, permutation, spectral, fuzzy, multiscale
  • Fractal dimensions: Katz, Higuchi, Petrosian, Sevcik, correlation dimension
  • Nonlinear dynamics: Lyapunov exponents, Lempel-Ziv complexity, recurrence quantification
  • DFA: Detrended fluctuation analysis, multifractal DFA
  • Information theory: Fisher information, mutual information

Key functions:

```python

# Multiple complexity metrics at once

complexity_indices = nk.complexity(signal, sampling_rate=1000)

# Specific measures

apen = nk.entropy_approximate(signal)

dfa = nk.fractal_dfa(signal)

lyap = nk.complexity_lyapunov(signal, sampling_rate=1000)

```

10. Event-Related Analysis

Create epochs around stimulus events and analyze physiological responses. See references/epochs_events.md for workflows.

Primary capabilities:

  • Epoch creation from event markers
  • Event-related averaging and visualization
  • Baseline correction options
  • Grand average computation with confidence intervals

Key functions:

```python

# Find events in signal

events = nk.events_find(trigger_signal, threshold=0.5)

# Create epochs around events

epochs = nk.epochs_create(signals, events, sampling_rate=1000,

epochs_start=-0.5, epochs_end=2.0)

# Average across epochs

grand_average = nk.epochs_average(epochs)

```

11. Multi-Signal Integration

Process multiple physiological signals simultaneously with unified output. See references/bio_module.md for integration workflows.

Key functions:

```python

# Process multiple signals at once

bio_signals, bio_info = nk.bio_process(

ecg=ecg_signal,

rsp=rsp_signal,

eda=eda_signal,

emg=emg_signal,

sampling_rate=1000

)

# Analyze all processed signals

bio_analysis = nk.bio_analyze(bio_signals, sampling_rate=1000)

```

Analysis Modes

NeuroKit2 automatically selects between two analysis modes based on data duration:

Event-related analysis (< 10 seconds):

  • Analyzes stimulus-locked responses
  • Epoch-based segmentation
  • Suitable for experimental paradigms with discrete trials

Interval-related analysis (β‰₯ 10 seconds):

  • Characterizes physiological patterns over extended periods
  • Resting state or continuous activities
  • Suitable for baseline measurements and long-term monitoring

Most *_analyze() functions automatically choose the appropriate mode.

Installation

```bash

uv pip install neurokit2

```

For development version:

```bash

uv pip install https://github.com/neuropsychology/NeuroKit/zipball/dev

```

Common Workflows

Quick Start: ECG Analysis

```python

import neurokit2 as nk

# Load example data

ecg = nk.ecg_simulate(duration=60, sampling_rate=1000)

# Process ECG

signals, info = nk.ecg_process(ecg, sampling_rate=1000)

# Analyze HRV

hrv = nk.hrv(info['ECG_R_Peaks'], sampling_rate=1000)

# Visualize

nk.ecg_plot(signals, info)

```

Multi-Modal Analysis

```python

# Process multiple signals

bio_signals, bio_info = nk.bio_process(

ecg=ecg_signal,

rsp=rsp_signal,

eda=eda_signal,

sampling_rate=1000

)

# Analyze all signals

results = nk.bio_analyze(bio_signals, sampling_rate=1000)

```

Event-Related Potential

```python

# Find events

events = nk.events_find(trigger_channel, threshold=0.5)

# Create epochs

epochs = nk.epochs_create(processed_signals, events,

sampling_rate=1000,

epochs_start=-0.5, epochs_end=2.0)

# Event-related analysis for each signal type

ecg_epochs = nk.ecg_eventrelated(epochs)

eda_epochs = nk.eda_eventrelated(epochs)

```

References

This skill includes comprehensive reference documentation organized by signal type and analysis method:

  • ecg_cardiac.md: ECG/PPG processing, R-peak detection, delineation, quality assessment
  • hrv.md: Heart rate variability indices across all domains
  • eeg.md: EEG analysis, frequency bands, microstates, source localization
  • eda.md: Electrodermal activity processing and SCR analysis
  • rsp.md: Respiratory signal processing and variability
  • ppg.md: Photoplethysmography signal analysis
  • emg.md: Electromyography processing and activation detection
  • eog.md: Electrooculography and blink analysis
  • signal_processing.md: General signal utilities and transformations
  • complexity.md: Entropy, fractal, and nonlinear measures
  • epochs_events.md: Event-related analysis and epoch creation
  • bio_module.md: Multi-signal integration workflows

Load specific reference files as needed using the Read tool to access detailed function documentation and parameters.

Additional Resources

  • Official Documentation: https://neuropsychology.github.io/NeuroKit/
  • GitHub Repository: https://github.com/neuropsychology/NeuroKit
  • Publication: Makowski et al. (2021). NeuroKit2: A Python toolbox for neurophysiological signal processing. Behavior Research Methods. https://doi.org/10.3758/s13428-020-01516-y